Business and Financial Affairs > Environmental Health and Safety > Biosafety Program > Biosafety Manual > Contents >

Environmental Health and Safety

SDSU Biosafety Manual

San Diego State University
BIOHAZARD CONTROL PROGRAM

Part XXIII:
Training

A continuous, on-the-job safety training program is essential to maintain safety awareness among the laboratory and support staff. Laboratory managers, with the assistance of the BSO and other resource persons, play the key role in staff training. The effectiveness of biosafety training, indeed all safety and health training, depends on management commitment, motivational factors, adequate initial job training, good communications, and ultimately the institution’s goals and objectives. The following are critical elements for an effective biosafety training program:

A. MODULE 1: GOOD LABORATORY PRACTICE FOR SUPPORT STAFF

B. MODULE 2: GOOD LABORATORY PRACTICE FOR SCIENTISTS AND TECHNICAL STAFF

C. MODULE 3: GOOD LABORATORY PRACTICE FOR SPECIALIST STAFF WHO HANDLE MICROORGANISMS IN RISK GROUP 3

A. MODULE 1: GOOD LABORATORY PRACTICE FOR SUPPORT STAFF

This module is for the following groups of laboratory support staff who do not normally have any laboratory training.

· Group 1

Janitorial staff who clean the premises; dispose of contaminated and other laboratory wastes.

· Group 2

Engineering and maintenance staff who service laboratory facilities; repair equipment.

· Group 3

Staff who receive and sort pathological material brought to the laboratory; open mail; handle request forms and laboratory records; pack infectious materials for mailing or shipping; drive vehicles that carry infectious materials.

· Group 4

Staff who wash and prepare glassware and other equipment; prepare and sterilize culture media and reagents.

Course contents

The nature of microorganisms and how they cause infections (all groups).
The type of work of the laboratory (all groups).
How to avoid infection in the laboratory; personal hygiene, protective clothing, eating and drinking; biohazard signs and restricted areas (all groups).
Use and limitations of disinfectants (all groups).
Operation, control and testing of autoclaves and incinerators (groups 2 and 4).
Hazards of particular equipment, e.g., BSCs, incubators, refrigerators, homogenizers and centrifuges (group 2).
Hazards of internal transport and of receiving specimens; how to pack infectious materials for mailing and shipping; emergency action to be taken in case of accident or spillage before the biosafety officer arrives (group 3).
Chemical, physical, mechanical, electrical and biological hazards (all groups).
The BSO and his or her duties; explanation of the individual worker's rights and responsibilities according to national and local regulations; need to report accidents and unusual occurrences; local security and fire precautions (all groups).
Simple first aid (all groups).

B. MODULE 2: GOOD LABORATORY PRACTICE FOR SCIENTISTS AND TECHNICAL STAFF

This module is for scientists and technical staff who work in basic laboratories - BSLs 1 and 2.

Course contents

Classification of microorganisms according to Risk Group (RG) on the basis of hazard.
Laboratory infections; how they occur and the routes and modes of infection.
Infections due to known accidents, e.g., accidental inoculation, spillage; prevention or minimization.
Infections due to airborne infectious particles; how these particles (aerosols) are released.
Measurement and control of aerosols; reduction of hazards by changing techniques and equipment.
Protective clothing, face and eye protection, personal hygiene, immunization.
Class II BSCs (Class III BSCs are unlikely to be used in these laboratories).
Universal precautions for handling blood and body fluids.
Disposal of infected laboratory wastes; principles and use of autoclaves and incinerators.
Chemical disinfection: limitations and policies.
Animal Housing: contamination control (in coordination with the campus veterinarian).
Chemical and carcinogenic hazards; chemical fume cupboards; hazardous analytical equipment.
Electrical and fire hazards.
First aid for laboratory (microbiologically related) accidents.
Emergency procedures.
Mailing and shipping of infectious materials.

C. MODULE 3: GOOD LABORATORY PRACTICE FOR SPECIALIST STAFF WHO HANDLE MICROORGANISMS IN RISK GROUP 3

This module is for specialized scientific, technical and safety staff who will handle microorganisms in Risk Group 3 (RG 3). Staff who have not received the proper training should not be given responsibility for microorganisms in RG 3.

Examples of RG 3 microorganisms can be found in the NIH publication, NIH Guidelines for Research Involving Recombinant DNA Molecules.

Course contents

RG 3 microorganisms: identity, associated diseases; clinical features; natural reservoirs, vectors; modes of spread, and prevention
Potential hazards of genetic manipulations and their relation to microorganisms in RG 3
Levels of containment for RG 3 microorganisms
BSL 3 procedures
Specialized clothing and hygiene facilities
Specialized equipment: Class III BSCs, testing control, training in use; double-door pass-through autoclaves; warning against full dependence on mechanical equipment that may give a false sense of security
Simple maintenance of electrical power, water, pressure equipment, etc., in areas where maintenance staff cannot immediately be admitted
Immunization
Emergency measures
Documentation of activities

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This page last updated January 24, 2012
Site contact: UCO Web Support


	Business and Financial Affairs > Environmental Health and Safety > Biosafety Program > Biosafety Manual > Contents >
	Environmental Health and Safety SDSU Biosafety Manual San Diego State University BIOHAZARD CONTROL PROGRAM Part XXIII: Training A continuous, on-the-job safety training program is essential to maintain safety awareness among the laboratory and support staff. Laboratory managers, with the assistance of the BSO and other resource persons, play the key role in staff training. The effectiveness of biosafety training, indeed all safety and health training, depends on management commitment, motivational factors, adequate initial job training, good communications, and ultimately the institution’s goals and objectives. The following are critical elements for an effective biosafety training program: A. MODULE 1: GOOD LABORATORY PRACTICE FOR SUPPORT STAFF B. MODULE 2: GOOD LABORATORY PRACTICE FOR SCIENTISTS AND TECHNICAL STAFF C. MODULE 3: GOOD LABORATORY PRACTICE FOR SPECIALIST STAFF WHO HANDLE MICROORGANISMS IN RISK GROUP 3 A. MODULE 1: GOOD LABORATORY PRACTICE FOR SUPPORT STAFF This module is for the following groups of laboratory support staff who do not normally have any laboratory training. · Group 1 Janitorial staff who clean the premises; dispose of contaminated and other laboratory wastes. · Group 2 Engineering and maintenance staff who service laboratory facilities; repair equipment. · Group 3 Staff who receive and sort pathological material brought to the laboratory; open mail; handle request forms and laboratory records; pack infectious materials for mailing or shipping; drive vehicles that carry infectious materials. · Group 4 Staff who wash and prepare glassware and other equipment; prepare and sterilize culture media and reagents. Course contents The nature of microorganisms and how they cause infections (all groups). The type of work of the laboratory (all groups). How to avoid infection in the laboratory; personal hygiene, protective clothing, eating and drinking; biohazard signs and restricted areas (all groups). Use and limitations of disinfectants (all groups). Operation, control and testing of autoclaves and incinerators (groups 2 and 4). Hazards of particular equipment, e.g., BSCs, incubators, refrigerators, homogenizers and centrifuges (group 2). Hazards of internal transport and of receiving specimens; how to pack infectious materials for mailing and shipping; emergency action to be taken in case of accident or spillage before the biosafety officer arrives (group 3). Chemical, physical, mechanical, electrical and biological hazards (all groups). The BSO and his or her duties; explanation of the individual worker's rights and responsibilities according to national and local regulations; need to report accidents and unusual occurrences; local security and fire precautions (all groups). Simple first aid (all groups). B. MODULE 2: GOOD LABORATORY PRACTICE FOR SCIENTISTS AND TECHNICAL STAFF This module is for scientists and technical staff who work in basic laboratories - BSLs 1 and 2. Course contents Classification of microorganisms according to Risk Group (RG) on the basis of hazard. Laboratory infections; how they occur and the routes and modes of infection. Infections due to known accidents, e.g., accidental inoculation, spillage; prevention or minimization. Infections due to airborne infectious particles; how these particles (aerosols) are released. Measurement and control of aerosols; reduction of hazards by changing techniques and equipment. Protective clothing, face and eye protection, personal hygiene, immunization. Class II BSCs (Class III BSCs are unlikely to be used in these laboratories). Universal precautions for handling blood and body fluids. Disposal of infected laboratory wastes; principles and use of autoclaves and incinerators. Chemical disinfection: limitations and policies. Animal Housing: contamination control (in coordination with the campus veterinarian). Chemical and carcinogenic hazards; chemical fume cupboards; hazardous analytical equipment. Electrical and fire hazards. First aid for laboratory (microbiologically related) accidents. Emergency procedures. Mailing and shipping of infectious materials. C. MODULE 3: GOOD LABORATORY PRACTICE FOR SPECIALIST STAFF WHO HANDLE MICROORGANISMS IN RISK GROUP 3 This module is for specialized scientific, technical and safety staff who will handle microorganisms in Risk Group 3 (RG 3). Staff who have not received the proper training should not be given responsibility for microorganisms in RG 3. Examples of RG 3 microorganisms can be found in the NIH publication, NIH Guidelines for Research Involving Recombinant DNA Molecules. Course contents RG 3 microorganisms: identity, associated diseases; clinical features; natural reservoirs, vectors; modes of spread, and prevention Potential hazards of genetic manipulations and their relation to microorganisms in RG 3 Levels of containment for RG 3 microorganisms BSL 3 procedures Specialized clothing and hygiene facilities Specialized equipment: Class III BSCs, testing control, training in use; double-door pass-through autoclaves; warning against full dependence on mechanical equipment that may give a false sense of security Simple maintenance of electrical power, water, pressure equipment, etc., in areas where maintenance staff cannot immediately be admitted Immunization Emergency measures Documentation of activities
	[top of page]	This page last updated January 24, 2012 Site contact: UCO Web Support

Environmental Health and Safety

SDSU Biosafety Manual

Part XXIII: Training

Part XXIII:
Training