Environmental Health and Safety

SDSU Biosafety Manual

San Diego State University
BIOHAZARD CONTROL PROGRAM

Part IX:
Human Cell and Tissue Culture

A. WORKING WITH HUMAN AND NON-HUMAN PRIMATE TISSUE CULTURES

B. WORKING WITH HUMAN AND NON-HUMAN PRIMATE CELL CULTURES

A. WORKING WITH HUMAN AND NON-HUMAN PRIMATE TISSUE CULTURES

Human and non-human primate blood, blood products, body fluids and tissues are listed as potentially hazardous biological materials.

No experiment shall be conducted on persons or human tissues unless specifically approved by the IRB and the IBC.

BSL 2 practices and procedures must be followed when handling human and non-human primate blood, blood products, body fluids and tissues because of the infectious agents they may contain. BSL 2 practices and procedures are consistent with the concept known as “Universal Precautions.”

B. WORKING WITH HUMAN AND NON-HUMAN PRIMATE CELL CULTURES

When cell cultures are known to contain an etiologic agent, an oncogenic virus or amphotropic packaging system, the cell line must be classified at the same BSL as that recommended for the agent itself. Furthermore, the following must be handled at BSL 2 or higher containment:

• All cell lines of human/non-human primate origin.
  
  
• All cell lines derived from lymphoid or tumor tissue.
  
  
• All cell lines exposed to or transformed by any oncogenic virus.
  
  
• All cell lines exposed to or transformed by amphotropic packaging systems.
  
  
• All cell lines new to the laboratory (until proven to be free of all adventitious agents).
  
  
• All cell lines containing Mycoplasmas.