SDSU Biosafety Manual


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	Environmental Health and Safety SDSU Biosafety Manual BIOHAZARD CONTROL PROGRAM AT SAN DIEGO STATE UNIVERSITY A Program for Compliance with CDC/NIH Guidelines on Biosafety in Laboratories, OSHA Bloodborne Pathogen Standard, DHHS/USDA Select Agent and Toxin Regulations, DOT Transportation of Hazardous Materials and the California Medical Waste Management Act Prepared by: Department of Environmental Health and Safety Revised: November 2003 Contents Acknowledgements Executive Summary Part I: Introduction Purpose, Scope and Policy Responsibilities Part II: Biological Use Authorization Application, Review and Approval Process A. Requirements for Review and Approval of Projects Using Infectious Agents and/or Recombinant DNA B. Requirements for Review and Approval of Transgenic Animal Projects C. Requirements For Review And Approval Of Select Agents And Toxins Projects D. Requirements For Submission Of A New Biological Use Authorization Application Attachment A: Frequently Asked Questions about the BUA Attachment B: Biological Use Authorization (BUA) Application Form and its Appendices Part III: Principles of Biosafety A. Terminology B. Laboratory Biosafety Level Criteria C. Vertebrate Animal Biosafety Level Criteria Part IV: Biosafety Level Practices A. Biosafety Level Practices Chart B. Biosafety Level 1 Practices C. Biosafety Level 2 Practices D. Biosafety Level 3 Practices Part V: Bloodborne Pathogen Control Plan A. Introduction and Overview B. Policy C. Responsibilities D. Exposure Control Plan Components E. Exposure Determination F. Methods of Compliance G. Special Hand\lking- HIV, HBV AND HCV Research Laboratories H. Hepatitis B Vaccinations and Post-Exposure Follow-Ups I. Hazard Communication J. Recordkeeping Part VI: Infectious Agents A. Laboratory-Acquired Infections B. Work with Infectious Agents C. Types of Infections D. Modes of Infection Part VII: Select Agents and Toxins A. Purposes B. Responsibilities C. Requirements D. The Program E. Security F. Definitions Part VIII: Recombinant DNA Technology A. Introduction B. Biological Expression Systems C. Properties of the Donor Organism and Cloned DNA D. Viral Vectors for Gene Transfer E. Transgenic and “Knock-Out” Animals F. Transgenic Plants G. Conclusion H. Biological Use Authorization and Registration Part IX: Human Cell and Tissue Culture A. Working with Human and Non-Human Primate Tissue Cultures B. Working with Human and Non-Human Primate Cell Cultures Part X: Animals in Research A. Use of Animals in Research B. Disposal of Biohazardous Animals and Animal Carcasses Part XI: Signs and Labels A. Signs B. Labels Part XII: Personal Protective Clothing and Equipment A. Eye Protection B. Laboratory Clothing C. Gloves D. Respirators Part XIII: Safety Equipment A. Biosafety Cabinets B. Chemical Fume Hoods C. Centrifuges D. Equipment that Creates Aerosols Part XIV: Whole Building Safety A. Access B. Ventilation (including air supply, exhaust and distribution) C. Centrifuges D. Emergency Equipment Part XV: Laboratory Clean Areas Part XVI: Laboratory Housekeeping Part XVII: Laboratory Moves A. Disposal and Decontamination of Biohazardous Waste B. Moving the Biosafety Cabinet Part XVIII: Transportation and Transfer of Biological Agents A. Introduction B. Transportation C. Transfer Part XIX: Medical Waste Management A. Purpose B. Definitions C. Responsibilities D. Requirements E. Summary of Requirements Part XX: Biohazard Spill and Emergency Response A. Biohazard Spill Cleanup Procedures Part XXI: Decontamination A. Definitions B. General Procedures C. Methods Part XXII: Laboratory Inspection A. Environmental Health and Safety Inspection B. Self-inspection C. Regulatory Agency Inspection Part XXIII: Training A. Module 1: Good Laboratory Practice for Support Staff B. Module 2: Good Laboratory Practice for Scientists and Technical Staff C. Module 3: Good Laboratory Practice for Specialist Staff Who Handle Microorganisms in Risk Group 3 Appendices Glossary of Terms
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Environmental Health and Safety

SDSU Biosafety Manual

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